Scanning Laser Confocal Microscope

The Leica TCS SP5 Confocal microscope (purchased 2/12)

Utilizing a unique tandem system, it has a conventional scanner that offers true confocal data acquisition while a resonance scanner option delivers additional highspeed sensitivity, especially useful for imaging live samples. High signal to noise ratios are characteristic of Leica’s unique system of fully adjustable detectors, resulting in efficient collection of emission signal. It is relatively straightforward to learn and to operate.

  • Anti-vibration system
  • Inverted microscope (DMI6000B-CS)
    • 100 W Halogen lamp for brightfield microscopy
  • Objectives:
    • 10x, dry, 0.3 NA, HCX PL Fluotar
    • 20x, dry, 0.7 NA, HCX PL APO CS
    • 40x, oil, 1.3 NA, HCX PL APO
    • 63x oil, 1.4 NA, HCX PL APO
    • 63x water, 1.2 NA, HCX PL APO
  • Fluorescent Microscopy
    • 50 W metal halide lamp
    • Filter cubes: I3 (fluorescein, long-pass), N2.1 (rhodamine, long-pass), A4 (ultraviolet, short-pass cube)
  • Differential Interference Contrast, DIC
  • Confocal lasers
    • UV, diode laser, 405 nm
    • Argon gas laser: 458, 476, 488, 514 nm
    • Diode laser, 561 nm
    • Diode laser, 633 nm
  • Resonance Scanner: invaluable for imaging living material quickly with less phototoxicity
  • Spectrophotometric Detectors
    • 2 Photomultiplier Tube Detectors, with ability to change bandwith in 3-5 nm steps.
    • 1 HyD Detector, hybrid between PMT and photocathode, doubles the sensitivity with improved signal to noise. Allows for some fluorescence quantification.
    • 2 more channels available for addition PMTs and/or HyDs
  • Acousto-optical Tunable Filter (AOTF)
    • Fast and smooth way of controlling laser intensity.
  • Licenses for FRAP (Fluorescence Recovery after Photobleaching) and FRET (Fluorescence Resonance Energy Transfer)
  • Tokai Hit Stage Top Incubator for imaging living cells over a period of time.

Atomic Force Microscope

The Digital Instruments Nansocope IIIa Controller, phase extender module

  • Anti-vibration system
  • Multimode Scanning Probe Microscope
    • OEM 30mm long working distance lens optical navigation digital video.
  • Scanning Tunneling Microscopy
    • picoAmpere sensitivity
    • Electro-chemical cell
  • Scanners:
    • 1.2 µm manually adjusted "A" scanner
    • 15 µm vertical engage "E" scanner
    • 150 µm vertical engage "J" scanner
  • Probe Holders
    • Tapping mode
    • Electrostatic Force mode
    • Force Modulation mode
  • Fluid cells
    • Contact mode
    • Tapping mode