Dept. of Microbiology and Immunology
University of Michigan Medical School, 2010-2012
Microbiology and the biology of infection
My lab studies how bacterial pathogens interact with their host cells and with other microbes. I am particularly interested in (i) strategies used by pathogens to avoid destruction by host innate intracellular defenses, and (ii) mechanisms by which nonpathogenic probiotic bacteria can mitigate pathogen infection.
Bacterial pathogenesis and host-pathogen interactions
REFEREED PUBLICATIONS (*undergraduate student author, **M.S. student author)
Tranchemontagne, Z.R.**, R.B. Camire*, V.J. O'Donnell, J. Baugh* and K.M. Burkholder. Staphylococcus aureus strain USA300 perturbs acquisition of lysosomal enzymes and requires phagosomal acidification for survival inside macrophages. Infection and Immunity. (In press)
Abuaita, B.H., K.M. Burkholder, B.R. Boles and M.X.D. O'Riordan. 2015. The endoplasmic reticulum stress sensor inositol-reducing enzyme 1α augments bacterial killing through sustained oxidant production. MBio. 6(4):e00705.
Burkholder, K.M., J. Perry, C. Wobus, H. Showalter, V. Kapuria, N. Donato and M.X.D. O'Riordan. 2011. A small molecule deubiquitinase inhibitor increases localization of iNOS to the macrophage phagosome and enhances bacterial killing. Infection and Immunity. 12:4850-7.
Mishra, K.K., Mendonca, M., Aroonnual, A., Burkholder, K.M. and Bhunia, A.K. 2011. Genetic organization and molecular characterization of the secA2 locus in Listeria species. Gene. 489:76-85.
Burkholder, K.M and Bhunia, A.K. 2010. Listeria monocytogenes uses Listeria Adhesion Protein (LAP) to promote bacterial transepithelial translocation, and induces expression of LAP receptor Hsp60. Infection and Immunity. 78: 5062-5073.
Jagadeesan B., Koo O.-K., Kim K.-P., Burkholder K.M., Mishra K., Aroonnual A., Bhunia A.K. 2010. LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria, promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species. Microbiology. 156: 2782-95.
Burkholder, K.M., Kim, K-P., Mishra, K., Medina, S., Hahm, B-K., Kim, H., and Bhunia, A.K. 2009. Expression of LAP, a SecA2-dependent secretory protein, is induced under anaerobic environment. Microbes and Infection. 11: 859-867.
Burkholder, K.M. and Bhunia, A.K. 2009. Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG. Gut Pathogens. 1: 14.
Burkholder, K.M., K.L. Thompson, T.A. Applegate and J.A. Patterson. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology and susceptibility to Salmonella Enteriditis colonization in broilers. Poultry Science. 87: 1734-1741.
Akin, D., J. Sturgis, K. Ragheb, D. Sherman, K.M. Burkholder, J.P. Robinson, A.K. Bhunia, S. Mohammed, and R. Bashir. 2007. Bacteria-mediated delivery of nanoparticles into cells. Nature Nanotechnology. 2: 441-449.
Kim, K.-P., B. Jagadeesan, K.M. Burkholder, Z.W. Jaradat, J.L. Wampler, A.A. Lathrop, M.T. Morgan, and A.K. Bhunia. 2006. Adhesion characteristics of Listeria adhesion protein (LAP)-expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiology Letters. 256: 324-332.
Burkholder, K.M. and M.X.D. O'Riordan. In press. Opportunistic pathogens of humans. In Hurst C (ed), Advances in Environmental Microbiology, Volume 3. The Rasputin effect: when commendals and symbionts become parasitic. Springer, New York, N.Y.
Burkholder, K.M. and A.K. Bhunia. 2013. Listeria monocytogenes and human Hsp60 - an invasive pairing, p 267-282. In Henderson B (ed), Moonlighting cell stress proteins in microbial infections. Springer, New York, NY.