Dept. of Microbiology and Immunology
University of Michigan Medical School
Microbiology and the biology of infection
My lab studies how bacterial pathogens infect and survive within the cells of their host. I am particularly interested in strategies used by pathogens to avoid destruction by host innate intracellular defenses.
Bacterial pathogenesis and host-pathogen interactions
Burkholder, K.M. and A.K. Bhunia. 2013. Listeria monocytogenes and human Hsp60 - an invasive pairing, p 267-282. In Henderson B (ed), Moonlighting cell stress proteins in microbial infections. Springer, New York, NY.
Burkholder, K.M., J. Perry, C. Wobus, H. Showalter, V. Kapuria, N. Donato and M.X.D. OÕRiordan. 2011. A small molecule deubiquitinase inhibitor increases localization of iNOS to the macrophage phagosome and enhances bacterial killing. Infection and Immunity. 12:4850-7.
Mishra, K.K., Mendonca, M., Aroonnual, A., Burkholder, K.M. and Bhunia, A.K. 2011. Genetic organization and molecular characterization of the secA2 locus in Listeria species. Gene. 489:76-85.
Burkholder, K.M and Bhunia, A.K. 2010. Listeria monocytogenes uses Listeria Adhesion Protein (LAP) to promote bacterial transepithelial translocation, and induces expression of LAP receptor Hsp60. Infection and Immunity. 78: 5062-5073.
Jagadeesan B., Koo O.-K., Kim K.-P., Burkholder K.M., Mishra K., Aroonnual A., Bhunia A.K. 2010. LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria, promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species. Microbiology. 156: 2782-95.
Burkholder, K.M., Kim, K-P., Mishra, K., Medina, S., Hahm, B-K., Kim, H., and Bhunia, A.K. 2009. Expression of LAP, a SecA2-dependent secretory protein, is induced under anaerobic environment. Microbes and Infection. 11: 859-867.
Burkholder, K.M. and Bhunia, A.K. 2009. Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG. Gut Pathogens. 1: 14.
Burkholder, K.M., K.L. Thompson, T.A. Applegate and J.A. Patterson. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology and susceptibility to Salmonella Enteriditis colonization in broilers. Poultry Science. 87: 1734-1741.
Akin, D., J. Sturgis, K. Ragheb, D. Sherman, K.M. Burkholder, J.P. Robinson, A.K. Bhunia, S. Mohammed, and R. Bashir. 2007. Bacteria-mediated delivery of nanoparticles into cells. Nature Nanotechnology. 2: 441-449.
Kim, K.-P., B. Jagadeesan, K.M. Burkholder, Z.W. Jaradat, J.L. Wampler, A.A. Lathrop, M.T. Morgan, and A.K. Bhunia. 2006. Adhesion characteristics of Listeria adhesion protein (LAP)-expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiology Letters. 256: 324-332.