Behavioral and Genotyping Core

Mission

The mission of the UNE Behavioral Core is to provide COBRE investigators and the broader UNE research community with the highest level of technical expertise, training, instrumentation and related services for assessing behavior in animals. Through utilization of these services, researchers and trainees will gain insight into the function of the nervous system and mechanisms of acute and chronic pain.

Location: 304 Stella Maris


Services

Behavioral 

Behavioral Testing

  • General Behavioral Phenotyping: Observational Screens, Motor Function
  • Pain/Sensory Testing: Thermal, Mechanical, Chemical
  • Additional Neurobehavioral Tests: Psychiatric, Addiction, Learning and Memory
  • Miscellaneous Systems: GI Transit, Cardiovascular and Respiration

Other Services:

  • Assistance choosing behavioral test and statistical tests for assessing results.
  • Training in surgical techniques for small animal surgeries.
  • Staff expertise includes surgical methods for producing pain models; dosing of drugs/anesthetics including central, systemic and localized administration; and tissue extractions.

Animal Care Training

For more information contact Ivy Bergquist at ibergquist@une.edu

Genotyping 

Genotyping Services is a component of the Behavioral Core that focuses on identifying the allele state of genes found in transgenic mouse lines. The core specializes in Hot-start PCR, Touchdown PCR, ARMs PCR, multiplex PCR and standard PCR protocols. The goal of Genotyping Services is to assist principal investigators in PCR to increase efficiency and reduce costs. By having Genotyping Services dedicated to only genotyping it allows laboratory staff to focus on the research projects and reduce cage costs due to a quick turnaround. Genotyping Services is a start to finish core, with the final product being the labeled gel image directly to the laboratory for their analysis.

Other services:

  • Primer Design and Optimization
  • gDNA Extraction using NaOH method

Mouse Colony Management is an important aspect of genetic research. To be compliant with OLAW guidelines the Principle Investigator must generate the fewest number of animals to complete the experiment. The goal of this service is to provide assistance in designing breeding strategies to meet this criteria.

Mouse Colony Management

For more information contact genotyping@une.edu

Publications

Havelin J, Imbert I, Cormier J, Allen J, Porreca F, King T. Central Sensitization and Neuropathic Features of Ongoing Pain in a Rat Model of Advanced Osteoarthritis. J Pain. 2016 Mar;17(3):374-82.

Zhang Y, Williams DA, Zaidi SA, Yuan Y, Braithwaite A, Bilsky EJ, Dewey WL, Akbarali HI, Streicher JM, Selley DE. 17-Cyclopropylmethyl-3,14β-dihydroxy-4,5α-epoxy-6β-(4'-pyridylcarboxamido)morphinan (NAP) Modulating the Mu Opioid Receptor in a Biased Fashion. ACS Chem Neurosci. 2016 Mar 16;7(3):297-304.

Warner E, Krivitsky R, Cone K, Atherton P, Pitre T, Lanpher J, Giuvelis G, Bergquist I, King T, Bilsky E, and Stevenson G. Evaluation of a Postoperative Pain-Like State n Motivated Behavior in Rats: Effects of Plantar Incision on Progressive-Ratio Food-Maintained Responding. Drug Dev Reserach. Dec 2015; 76(8):432–441.

Burman M, Bilsky E. FAAH inhibitor OL-135 disrupts contextual, but not auditory, fear conditioning in rats. Behav Brain Res. 2016 Jul 15;308:1-5.

Meng ID, Barton ST, Mecum NE, Kurose M. Corneal sensitivity following lacrimal gland excision in the rat. Invest Ophthalmol Vis Sci. 2015 May;56(5):3347-54.

Lefever M, Li Y, Anglin B, Muthu D, Giuvelis D, Lowery JJ, Knapp BI, Bidlack JM, Bilsky EJ, Polt R. Structural Requirements for CNS Active Opioid Glycopeptides. J Med Chem. 2015 Aug 13;58(15):5728-41.

Motyl KJ, DeMambro VE, Barlow D, Olshan D, Nagano K, Baron R, Rosen CJ, Houseknecht KL. Propranolol Attenuates Risperidone-Induced Trabecular Bone Loss in Female Mice. Endocrinology. 2015 Jul;156(7):2374-83.

Podolsky AT, Sandweiss A, Hu J, Bilsky EJ, Cain JP, Kumirov VK, Lee YS, Hruby  VJ, Vardanyan RS, Vanderah TW. Novel fentanyl-based dual μ/δ-opioid agonists for the treatment of acute and chronic pain. Life Sci. 2013 Dec 18;93(25-26):1010-6.

Burman MA, Simmons CA, Hughes M, Lei L. Developing and validating trace fear conditioning protocols in C57BL/6 mice. J Neurosci Methods. 2014 Jan 30;222:111-7. 

Li Y, St Louis L, Knapp BI, Muthu D, Anglin B, Giuvelis D, Bidlack JM, Bilsky EJ, Polt R.

Can amphipathic helices influence the CNS antinociceptive activity of glycopeptides related to β-endorphin? J Med Chem. 2014 Mar 27;57(6):2237-46.

Mclane V, Cao L, Willis C. Morphine increases hippocampal viral load and suppresses frontal lobe CCL5 expression in the LP-BM5 AIDS model. J Neuroimmunol. 2014 Apr 15;269(1-2):44-51.

Burman M,  Erickson K,  Deal A,  Jacobson R, Contextual and Auditory Fear Conditioning Continue to Emerge during the Periweaning Period in Rats

Eur J Pharmacol. 2014 Aug 5;736:124-30.

Please remember to acknowledge the COBRE Behavioral Core and the financial support provided by the NIGMS (grant number P20GM103643) in manuscripts, grants and presentations that include our work.