Histology and Imaging Core

Mission

The mission of the Histology and Imaging Core is to provide COBRE investigators and the greater UNE research community access to expertise, training and specialized instrumentation related to tissue processing, sectioning, staining, immunohistochemistry and microscopy. 

Additionally the Core offers services and training related to image analysis and image analysis software to guide investigators in choosing the best methods for presenting their data. We also accommodate work for outside facilities on a fee-for-service basis.

Location: Pickus 219/220


Services

The Histology and Imaging Core is fully equipped to perform a wide range of histological processing to suit investigators’ needs. The Core also provides training to staff and students on equipment and techniques, including:

  • Trimming of wet tissues
  • Tissue processing into paraffin
  • OCT and paraffin embedding
  • Sectioning of paraffin-embedded/frozen tissues
  • Routine and special histochemical staining
  • Immunohistochemistry/Immunofluorescence
  • Antibody optimization
  • Brightfield/ widefield/ confocal microscopy
  • Image capture and image analysis

Training

The Core has cryostats, microtomes and microscopes available for reservation. To avoid scheduling conflicts and maintenance issues, all equipment must be reserved using Outlook calendars and individual users must be trained by Core staff.

The Leica confocal has a separate training procedure consisting of two 2-hour supervised sessions, several informational videos and an online quiz. The first training session is an introduction to the microscope guided by Core staff. The second session entails imaging of a user generated sample with minimal intervention from Core staff. Upon completion of a proficiency quiz, users can reserve time as desired.


Publications citing the Histology and Imaging Core

Havelin J, Imbert I, Cormier J, Allen J, Porreca F, King T. Central Sensitization and Neuropathic Features of Ongoing Pain in a Rat Model of Advanced Osteoarthritis. J Pain. 2016 Mar;17(3):374-82.

Deal A, Erickson K, Shiers S, Burman M, Limbic system development underlies the emergence of classical fear conditioning during the 3rd and 4th weeks of life in the rat. Behavioral Neuroscience. Behav Neurosci. 2016 Apr;130(2):212-30.

Warner E, Krivitsky R, Cone K, Atherton P, Pitre T, Lanpher J, Giuvelis G, Bergquist I, King T, Bilsky E, and Stevenson G. Evaluation of a Postoperative Pain-Like State n Motivated Behavior in Rats: Effects of Plantar Incision on Progressive-Ratio Food-Maintained Responding. Drug Dev Reserach. Dec 2015; 76(8):432–441.

Burman M, Bilsky E. FAAH inhibitor OL-135 disrupts contextual, but not auditory, fear conditioning in rats. Behav Brain Res. 2016 Jul 15;308:1-5.

Burman MA, Simmons CA, Hughes M, Lei L. Developing and validating trace fear conditioning protocols in C57BL/6 mice. J Neurosci Methods. 2014 Jan 30;222:111-7. 

Mclane V, Cao L, Willis C. Morphine increases hippocampal viral load and suppresses frontal lobe CCL5 expression in the LP-BM5 AIDS model. J Neuroimmunol. 2014 Apr 15;269(1-2):44-51.

Please remember to acknowledge the COBRE Histology and Imaging Core and the financial support provided by the NIGMS (grant number P20GM103643) in manuscripts, grants and presentations that include our work.

For a complete publication list, visit My NCBI.

Histology and Imaging Core