About the In Vitro Analytical Core
The in Vitro Analytical Core (IVAC) facilitates the mission of the UNE Center for Cell Signaling Research (CCSR) by identifying molecular mechanisms and cellular processes in aging, stress, bioenergetics, and cellular metabolism by providing the critical expertise and instrumentation to study cell-cell interactions and to delineate intracellular pathways in targeted cell types.
The primary mission of the IVAC is to support the research of current and future CCSR Research Project Leaders. As a resource at UNE, the IVAC plans to grow its capacity to provide services to all investigators at UNE, and ultimately to extramural investigators from both academic and industry partners in the region.
Services
The IVAC provides training, instrumentation, and technical services for the development, analysis, and storage of in vitro cell models (such as stably transfected cell lines using genetically encoded biosensors) across a wide range of biological and biomedical applications, as well as various types of sample preparation for downstream analyses such as mass spectrometry and RNA-seq.
These images depict a range of cell lines and primary neurons derived from mouse, rat and human, produced by members of the CCSR. The IVAC provides equipment and services for propagating, transfecting, analyzing and storing cell lines for a wide range of imaging and biochemical assays, as well as for preparation of samples for -omics applications.
Equipment
Cell Culture
- Two cell culture suites with biosafety cabinets, CO2 incubators
- BioRad ZOE Fluorescent Cell Imager
- Leica DM IL Inverted LED Fluorescent Microscope with a K3M camera
- Zeiss Dissecting Microscopes
- BioRad TC Automated Cell Counter
- Thermo Scientific Sorvall ST4 Plus refrigerated centrifuge
- Lonza 4D-nucleofector
- Biolife V-3000AB isothermal Liquid Nitrogen freezer
Molecular Biology
- Electrophoresis and Immunoblotting equipment
- Mystaire PCR hoods
- NanoDrop One C
- Agilent Bioanalyzer
- Eppendorf 5910Ri refrigerated centrifuge
- Eppendorf Mastercycler Pro (2x 96-well) and Mastercycler Pro S
- Eppendorf Thermomixer C
- FujiFilm Gel-doc System
- Typhoon FLA 9500 laser gel and blot scanner
High-throughput Sample Processing and Analysis
- CellInsight CX7 LZR high content screening platform
- QuantStudio 3 RT-PCR
- Applied Biosystems StepOne Plus RT-PCR system
- Luminex® MagPix for multiplex protein quantification
- Molecular Devices SpectraMax M5 plate reader
- Molecular Devices Fluorescent Imaging Plate Reader (FLIPR®) Tetra high-throughput cellular screening system
- Life Technologies Multi-Drop Combi Plus reagent dispenser
- E1-ClipTip Electronic Adjustable Tip Spacing Multi-Channel pipettes (1250 & 125 µl)
General laboratory equipment includes: benchtop centrifuges, balances, stir plates, vortex mixers, shakers, rockers, incubated shakers, hot plates and hot blocks, single- and multichannel pipettes, and an HPC refrigerator.
Researchers can schedule time to use the IVAC equipment and facilities via iLab at https://my.ilabsolutions.com/account/login.
To coordinate a training time or if you need access to iLab, contact us via email at ivac@une.edu.
Contact
Core Email
How to Cite Use
The UNE In Vitro Analytical Core is supported by the UNE Center for Cell Signaling Research through an Institutional Development Award (IDeA) from the National Institutes of Health (P20GM152330). The IVAC also has an ABRF (Association of Biomolecular Resource Facilities) account and is listed on the Core Marketplace with the Research Resource Identifier (RRID): SCR_026905 (https://coremarketplace.org/UNEIVAC).
The full citation is: “University of New England - In Vitro Analytical Core, RRID: SCR_026905, 5P20GM152330-02”. This is also listed on the ABRF website for the core (https://coremarketplace.org/UNEIVAC).
If you need additional information for preparing a grant application, please feel free to contact the IVAC directly.