Equipment

• Leica RM2265 fully automated rotary microtome
• Leica Autostainer XL automated stainer
• Sakura TEC 5 embedding center
• Sakura VIP E150 automated tissue processor
• Leica VT1000S vibrating microtome
• Leica CM1950 and CM1900 cryostats
• Biocare Medical Decloaking chamber for heat-induced epitope retrieval
• Electrophoretic tissue clearing system for CLARITY technique

Protocols and Resources

• A plethora of knowledge surrounding the chemistry of fixation, and histological dyes
• Nikon’s online source for microscopy knowledge
• Dako IHC Handbook — everything you need to know about IHC
• Calculation of proper sampling parameters